With 20 microliter pipette, remove all the remaining ethanol from the bottom of the tube.Perform two wash steps while keeping the tubes in the magnetic rack:.The desired DNA is absorbed on the beads. The supernatant contains the short DNA and the low molecular weight impurities. Pipette the supernatant out while keeping the tubes on the magnetic rack.Incubate on bench for 5-15 minutes, so the DNA absorbs on the beads.In our example, add 50 - 30 = 20 microliters of the magnetic beads suspension To the tubes with the supernatant, add the magnetic beads suspension for the shorter DNA removal.Using our example, the supernatant contains DNA shorter than 600 bp. Transfer the supernatant to the newly labelled tubes.Label new tubes and place them in a tube rack.Wait for the solution to clear (30 seconds - 2 minutes).Place the sample into the magnetic rack.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |